It is well accepted that seawater injection is able to improve the water wetness of carbonate reservoirs at high temperatures, and in that way, it can act as an enhanced oil recovery (EOR) fluid. A recent laboratory investigation showed that increased oil recovery also was obtained from carbonate reservoir cores by successively flooding composite limestone cores by 2, 10, and 20 times diluted seawater. The study confirmed that it is possible to obtain low salinity EOR effects also in carbonates, and not only in sandstones. In the present study, preserved reservoir core material from a similar limestone formation was used with the objective to obtain a chemical understanding of the mechanism for the improved oil recovery. It was verified, that the core material contained significant amounts of anhydrite (CaSO4), which appeared to be the key factor for observing the low salinity EOR effect. The concentration of sulfate in the injection brine increased due to increased dissolution of anhydrite as the salinity and concentration of inactive salt, NaCl, decreased. Both an increase in the sulfate concentration and a decrease in NaCl content in the injected brine will have a positive effect on the wettability alteration process. An oil displacement test was conducted on a restored reservoir core at 100 degrees C using 100 times diluted formation water in a tertiary flooding process, after first injecting formation water. This showed a low salinity EOR effect of 22% of original oil in place (OOIP), corresponding to an 88% increase in oil recovery. Also 30 times diluted seawater increased the oil recovery by 18% of OOIP in a tertiary flood after first flooding the core with formation water. After flooding a core successively at 100 degrees C with formation water, seawater, and 10 times diluted seawater, the oil recovery increased gradually by 25, 30, and 33% of OOIP. The chemical low salinity EOR mechanism was discussed in terms of dissolution of anhydrite and a decrease in the NaCl concentration. This wettability alteration mechanism is, in principle, the same as that reported previously for injection of seawater and modified seawater into chalk cores, involving a symbiotic interaction between Ca2+, Mg2+, and SO42- at the rock surface. In this case, supply of extra Ca2+ and SO42- was obtained by dissolution of anhydrite. The low salinity EOR technique can have a great economic potential regarding oil recovery from high temperature carbonate reservoirs containing significant amounts of dissolvable anhydrite distributed in the pore space.