The timing of petroleum generation of source rocks is recognized to investigate the maturation level of the proven potential source formations for oil preservation dead lines, and predict the levels of thermal maturity of the studied sequences in terms of hydrocarbon generation and expulsion. For theses purposes four thermal burial history and maturity profiles were constructed of the Abu Gharaig-1 SW Umbaraka-1, GPZ-1, and GPX-1 wells. The results showed Abu Roash and Bahariya formations are immature to mature source rocks and have poor to fair potential to generate both oil and gas and still are within the early stage of hydrocarbon generation till the present time. Alam El Bueib and Masajid formations bear mature source rocks and have good generating capability for generating both oil and gas and entered the oil window since Cretaceous to Miocene times and lies within oil and gas windows at present time. Khatatba Formation bears a mature source rock and has good generating capability for both oil and gas and entered the oil window during Late Cretaceous to Eocene times.