In this study, Ca-Mg-Al hydrotalcite was deposited on the inert honeycomb ceramic surface as a second carrier and KF was loaded on the support as an active component. The KF/Ca-Mg-Al hydrotalcite/honeycomb ceramic monolithic catalyst was packed in the membrane reactor for biodiesel production from the transesterification between soybean oil and methanol. The central composite design (CCD) of response surface methodology (RSM) was employed to investigate the effects of reaction temperature, catalyst amount and circulation velocity on the production of biodiesel. A quadratic model was developed to navigate the design space. The optimum condition for the highest biodiesel yield was obtained by the assistance of the model. The catalyst showed a reasonable efficiency and reusability for coupling the monolithic catalyzed transesterification and membrane separation in the membrane reactor. (C) 2015 Elsevier Ltd. All rights reserved.