Involvement of the yciW gene in L-cysteine and L-methionine metabolism in Escherichia coli

Kawano Y; Ohtsu I; Tamakoshi A; Shiroyama M; Tsuruoka A; Saiki K; Takumi K; Nonaka G; Nakanishi T; Hishiki T; Suematsu M; Takagi H

Journal of Bioscience and Bioengineering, Vol.119, No.3, 310-313, 2015

DOI10.1016/j.jbiosc.2014.08.012 Export Citation

Involvement of the yciW gene in L-cysteine and L-methionine metabolism in Escherichia coli

Kawano Y, Ohtsu I, Tamakoshi A, Shiroyama M, Tsuruoka A, Saiki K, Takumi K, Nonaka G, Nakanishi T, Hishiki T, Suematsu M, Takagi H

We here analyzed a sulfur index of Escherichia coli using LC-MS/MS combined with thiol-specific derivatization by monobromobimane. The obtained sulfur index was then applied to evaluate the L-cysteine producer. E. coli cells overexpressing the yciW gene, a novel Cys regulon, accumulated L-homocysteine, suggesting that YciW is involved in L-methionine biosynthesis. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.

Keywords:Escherichia coli;L-Cysteine;Metabolome analysis;Monobromobimane;yciW gene