Lipase-catalyzed glycerolysis of olive oil in organic solvent medium:Optimization using response surface methodology

Abhishek Kumar Singh; Singh and Mausumi Mukhopadhyay

Korean Journal of Chemical Engineering, Vol.33, No.4, 1247-1254, April, 2016

DOI10.1007/s11814-015-0272-y Export Citation

Lipase-catalyzed glycerolysis of olive oil in organic solvent medium:Optimization using response surface methodology

Abhishek Kumar Singh, and Singh and Mausumi Mukhopadhyay^†

Department of Chemical Engineering, Sardar Vallabhbhai National Institute of Technology, Surat 395007, Gujarat, India

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Enzymatic glycerolysis of olive oil for mono- (MG) and diglycerides (DG) synthesis was investigated. Several pure organic solvents and co-solvent mixtures were screened in a batch reaction system. The yields of MG and DG in co-solvent mixtures exceeded those of the corresponding pure organic solvents. Batch reaction conditions of the glycerolysis reaction, the lipase amount, the glycerol to oil molar ratio, the reaction time, and temperature, were studied. In these systems, the high content of reaction products, especially MG (55.8 wt%) and DG (16.4wt%) was achieved at 40 oC temperature and 0.025 g of lipase with relatively low glycerol to oil molar ratio (2 : 1) within 4 h of reaction time in isopropanol/tert-butanol (1 : 3) solvent mixture. Glycerolysis reaction was optimized with the assistance of response surface methodology (RSM). Optimal condition for reaction conversion was recommended as lipase amount 0.025 g, glycerol to oil molar ratio 2 : 1, reaction time 4 h and temperature 40 ℃.

Keywords:Monoglycerides;Immobilized Candida rugosa Lipase;Glycerolysis;Response Surface Methodology;Olive Oil

[References]

Blasi F, Cossignani L, Simonetti MS, Damiani P, Enzyme Microb. Technol., 41(6-7), 727 (2007)
Singh AK, Mukhopadhyay M, Appl. Biochem. Biotechnol., 166(2), 486 (2012)
Singh AK, Mukhopadhyay M, Korean J. Chem. Eng., 31(7), 1225 (2014)
Kase M, Moriwaki J, Shimizu M, US Patent, No. 92650 (2010).
Plou FJ, Barandiaran M, Calvo MV, Ballesteros A, Pastor E, Enzyme Microb. Technol., 18(1), 66 (1996)
Singh AK, Mukhopadhyay M, Grasas Aceites, 63, 202 (2012)
Pawongrat R, Xu X, Kittikun AH, Food Chem., 104, 251 (2007)
Damstrup ML, Abildskov J, Kiil SZ, Jensen AD, Sparso FV, Xu X, J. Agric. Food Chem., 54, 7113 (2006)
Jackson MA, King JW, J. Am. Oil Chem. Soc., 74, 103 (1997)
Guo Z, Xu X, Green Chem., 8, 54 (2006)
Rendon X, Lopez-Munguia A, Castillo E, J. Am. Oil Chem. Soc., 78, 1061 (2001)
AkohCC, Cooper C, Nwosu CV, J. Am. Oil Chem. Soc., 69, 257 (1992)
Bellot JC, Choisnard L, Castillo E, Marty A, Enzyme Microb. Technol., 28(4-5), 362 (2001)
Kaewthong W, H-Kittikun A, Enzyme Microb. Technol., 35(2-3), 218 (2004)
Li ZY, Ward OP, J. Am. Oil Chem. Soc., 70, 745 (1993)
Ghamgui H, Miled N, Rebai A, Karra-chaabouni M, Gargouri Y, Enzyme Microb. Technol., 39(4), 717 (2006)
Fang X, Xie N, Chen X, Yu H, Chen J, Food Bioprod Process, 90, 676 (2012)
Singh AK, Mukhopadhyay M, Chem. Ind. Chem. Eng. Q., 20, 127 (2014)
Fregolente LV, Batistella CB, Filho RM, Maciel MRW, J. Am. Oil Chem. Soc., 82, 673 (2005)
Zheng Y, Wu XM, Branford-White C, Quan J, Zhu LM, Bioresour. Technol., 100(12), 2896 (2009)
Fu BY, Vasudevan PT, Energy Fuels, 23(8), 4105 (2009)
Laane C, Boeren S, Vos K, Veeger C, Biotechnol. Bioeng., 30, 81 (1987)
Fu B, Vasudevan PT, Energy Fuels, 24, 4646 (2010)
Tuter M, Aksoy HA, Biotechnol. Lett., 22(1), 31 (2000)
Yang T, Rebsdorf M, Engelrud U, Xu X, J. Food Lip., 12, 299 (2005)
Yamane T, Kang ST, Kawahara K, Koizumi Y, J. Am. Oil Chem. Soc., 71, 339 (1994)

[Referenced By]

[1] Blasi F, Cossignani L, Simonetti MS, Damiani P, Enzyme Microb. Technol., 41(6-7), 727 (2007)

[2] Singh AK, Mukhopadhyay M, Appl. Biochem. Biotechnol., 166(2), 486 (2012)

[3] Singh AK, Mukhopadhyay M, Korean J. Chem. Eng., 31(7), 1225 (2014)

[4] Kase M, Moriwaki J, Shimizu M, US Patent, No. 92650 (2010).

[5] Plou FJ, Barandiaran M, Calvo MV, Ballesteros A, Pastor E, Enzyme Microb. Technol., 18(1), 66 (1996)

[6] Singh AK, Mukhopadhyay M, Grasas Aceites, 63, 202 (2012)

[7] Pawongrat R, Xu X, Kittikun AH, Food Chem., 104, 251 (2007)

[8] Damstrup ML, Abildskov J, Kiil SZ, Jensen AD, Sparso FV, Xu X, J. Agric. Food Chem., 54, 7113 (2006)

[9] Jackson MA, King JW, J. Am. Oil Chem. Soc., 74, 103 (1997)

[10] Guo Z, Xu X, Green Chem., 8, 54 (2006)

[11] Rendon X, Lopez-Munguia A, Castillo E, J. Am. Oil Chem. Soc., 78, 1061 (2001)

[12] AkohCC, Cooper C, Nwosu CV, J. Am. Oil Chem. Soc., 69, 257 (1992)

[13] Bellot JC, Choisnard L, Castillo E, Marty A, Enzyme Microb. Technol., 28(4-5), 362 (2001)

[14] Kaewthong W, H-Kittikun A, Enzyme Microb. Technol., 35(2-3), 218 (2004)

[15] Li ZY, Ward OP, J. Am. Oil Chem. Soc., 70, 745 (1993)

[16] Ghamgui H, Miled N, Rebai A, Karra-chaabouni M, Gargouri Y, Enzyme Microb. Technol., 39(4), 717 (2006)

[17] Fang X, Xie N, Chen X, Yu H, Chen J, Food Bioprod Process, 90, 676 (2012)

[18] Singh AK, Mukhopadhyay M, Chem. Ind. Chem. Eng. Q., 20, 127 (2014)

[19] Fregolente LV, Batistella CB, Filho RM, Maciel MRW, J. Am. Oil Chem. Soc., 82, 673 (2005)

[20] Zheng Y, Wu XM, Branford-White C, Quan J, Zhu LM, Bioresour. Technol., 100(12), 2896 (2009)

[21] Fu BY, Vasudevan PT, Energy Fuels, 23(8), 4105 (2009)

[22] Laane C, Boeren S, Vos K, Veeger C, Biotechnol. Bioeng., 30, 81 (1987)

[23] Fu B, Vasudevan PT, Energy Fuels, 24, 4646 (2010)

[24] Tuter M, Aksoy HA, Biotechnol. Lett., 22(1), 31 (2000)

[25] Yang T, Rebsdorf M, Engelrud U, Xu X, J. Food Lip., 12, 299 (2005)

[26] Yamane T, Kang ST, Kawahara K, Koizumi Y, J. Am. Oil Chem. Soc., 71, 339 (1994)