Hybrid protein crystals exhibiting enhanced photoluminescence were obtained by incorporation of bromophenol blue dye molecules within the organized solvent channels of cross-linked lysozyme single crystals. From the FT-IR, TEM, EDX, UV-Vis, and confocal characterization, we show that the BpB dye is inside the solvent channels of the crystals which has been bound up with the Trp62 and Trp108 residues of the protein.