TiO2/MMT nanocomposite was synthesized and characterized by X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), Energy-dispersive Xray spectroscopy (EDX), transmission electron microscopy (TEM), X-ray fluorescence (XRF) and Brunauer-Emmett-Teller (BET) techniques. The average size of TiO2 nanoparticles was decreased from 60-80 nm to 40-60nm through the immobilization on MMT. The main influential factors such as the TiO2/MMT dose, ciprofloxacin (CIP) concentration, pH of the solution, UV light regions, reusability of the catalyst and electrical energy determination were studied. The addition of radical scavengers (e.g. chloride, iodide, sulfate and bicarbonate) and enhancers (e.g. hydrogen peroxide, potassium iodate and peroxydisulfate) on the degradation efficiency was studied. The predicted data from the designed artificial neural network model were found to be in a good agreement with the experimental data (R-2=0.9864). The main intermediates of CIP degradation were determined by GC-Mass spectrometry. (C) 2015 Elsevier B.V. All rights reserved.