The aim of the present study was to control entanglements in order to regulate the properties of polymeric solids. Initially, fabrication of polymeric solids with few entanglements was attempted. Films of the DNA-cationic surfactant, cetyltrimethylammonium bromide (CTAB) (DNA-CTA), were cast from ethanol solution at room temperature. Morphological examination of DNA-CTA complex films using atomic force microscopy (AFM) revealed that these films were constructed by particle-like substances. Geometrical analysis of AFM images showed that the particle-like substances were the aggregates of several DNA-CTA globules. Mechanical characterization suggested that there were fewer entanglements than with normal plastic films. Small angle X-ray scattering experiments during annealing indicated that molecular motions were highly excited in the surface region of each particle. In conclusion, a globular polymeric film with fewer entanglements was fabricated. (C) 2015 Wiley Periodicals, Inc.