Herein, we synthesized a series of 10 core-shell silver-silica nanoparticles with a photosensitizes, Rose Bengal, tethered to their surface. Each nanoparticle possesses an identical silver core of about 67 nm, but presents a different silica shell thickness ranging from 5 to 100 am. These hybrid plasmonic nanoparticles thus afford a plasmonic nanostructure platform with a source,of singlet oxygen (O-1(2)) at a well-defined distance from the metallic core. Via time-resolved and steady state spectroscopic techniques, we demonstrate the sillier core exerts a= dual role of enhancing both the production of O-1(2), through enhanced absorption of light, and its radiative decay, which in turn boosts O-1(2) phosphorescence emission to a greater extent. Furthermore, we show both the production arid emission of O-1(2), in vitro to be dependent on proximity to the plasmonic nanostructure. Our results clearly exhibit three distinct regimes as the plasmonic nanostructure moves apart from the O-1(2) source, with a greater enhancement for silica shell thicknesses ranging between 10 and 20 nm. Moreover, these hybrid plasmonic nanoparticles can be delivered to both Gram-positive and Gram-negative bacteria boosting both photoantibacterial activity and detection limit of O-1(2) in cells.