The recovery of iron oxides from acid mine drainage (AMD) has attracted extensive research attention due to the double advantage of waste minimization and resource recovery. Recently a novel air-cathode fuel cell approach was proposed to in-situ utilize ferrous iron (Fe(II)) in the AMD for the fabrication of Fe3O4/graphite felt (GF) composite as the cathode of electro-Fenton process. In the present work, the influence of fuel cell operating parameters, including solution pH, carbonate concentration and Fe(II) concentration, on the catalytic activity of prepared Fe3O4/GF composite is adequately elucidated. The highest activity is observed on the composite obtained from the fuel cell operated with 30 mM Fe(II) and 50 mM carbonate at pH 7.5. The activity of Fe3O4/GF is strongly dependent upon iron loading and Fe3O4 morphology in the composite. Higher iron loading generally induces higher catalytic activity, and the Fe3O4 aggregate is catalytically less reactive relative to the well-dispersed one. The precipitation of Fe(III) oxides on the GF through electrochemical oxidation of Fe(II) plays a key role in determining the structure of Fe3O4/GF composite. Solution pH and composition in the fuel cell affect such a process by manipulating the distribution of Fe(II) species in aqueous solution and on the GF. (C) 2015 Elsevier Ltd. All rights reserved.