The concession area of the Jordan Oil Shale Energy Co (JOSE) is located in the southern border zone of the Attarat Um Ghudran deposit, next to the Wadi Maghara deposit, both consisting of marinite type oil shale (OS). These deposits of the Upper Cretaceous to Lower Paleogene Muwaqqar Chalk-Marl Formation form a huge north-southward elongated oil shale basin in Central Jordan, with resources over 55 billion tons. JOSE has drilled a regular grid of boreholes with a full coring of the up to 90 m thick OS seam and its lower and upper contact layers. Visually, the OS unit is a rather homogenous dark-colored (grey, black, brownish grey) succession of finely bedded (laminated) kerogen-bearing carbonate rocks that has been in earlier papers described as a uniform lithological unit. The aim of the geological and lithological studies of the JOSE exploration area was (i) to investigate the vertical variation of OS composition and, if present, to define layers within the OS unit, and (ii) to identify lithological varieties and chemical composition of OS present in different layers. On the basis of field evidence, downhole gamma-logging, chemical analyses and other criteria, an original detailed scheme of the layered structure of oil shale and barren rocks was introduced. A total of eight OS layers (indexed as A, B1, B2, C, D, E1, E2, E3) and at least four barren dolomitic limestone interlayers were distinguished. The present publication is dedicated to the chemical study of the layers and the total OS seam. A representative gapless collection of 632 conventional core samples from 12 cores serves as the base for the comparative study of the layers. Two main (SiO2, CaO) and two subordinate chemical (Al2O3 and P2O5) components of the mineral matter (MM), and loss on ignition (LOI 500 degrees C) approximately reflecting the content of organic matter (OM), are the basic variables discussed. Contents of SiO2 and CaO always show negative correlation, whereas local enrichment with Al2O3 and P2O5 occurs in certain interbeds. OM content in samples has no strong correlation with mineral matter abundances. The eight distinguished OS layers comprise both those strongly enriched in CaO, or oppositely in SiO2. The layers differ in rate of internal heterogeneity reflected in variation of standard deviation values. With rare exceptions, the barren limestone interlayers are dolomitized, strongly enriched with MgO and depleted of CaO. The database on the distribution of mineral compounds and trace elements serves for the 3-D block modelling of the deposit composition. However, further data analysis is required for the understanding of lateral changes of the layers' mineral composition, and geological and geochemical structure.