Currently available methods to prepare conducting polymers-coated colloidal substrates for biomedical applications need to be improved because they involve the use of toxic reagents and tend to result in aggregated products with diminished conductivity. The work herein describes for the first time a facile strategy for preparing highly water-dispersible, highly conductive, and biocompatible polypyrrole-coated silica core-shell (SiO2@PPy) particles using only chondroitin sulfate (CS), a biologically derived polymer, as the stabilizer and dopant. The CS preadsorbed onto silica surface serves as a template to control the confined growth of the PPy shell and doping of in situ polymerized PPy shell. The thickness of the PPy shell can be tuned from 8 to 17 nm by varying the CS preadsorbed amount. Increasing the thickness of the adsorbed CS layer can control the deposition of thinner PPy shells on an SiO2 core surface to provide highly water-dispersible SiO2@PPy particles. Moreover, CS-doped SiO2@PPy particles exhibit conductivities as high as 5.3 S cm(-1). The conductivity of the particles depends on the PPy mass loading and the doping level of the PPy shell. Furthermore, the SiO2@PPy particles exhibit good biocompatibility and therefore have potential applications in biomedicine.