The continuous removal of lead from aqueous solutions by Ca(II) imprinted chitosan (Ca(II)-CS) microspheres packed column is carefully investigated in this work. The modified dose-response model and the Thomas model are exploited to evaluate dynamic behaviors of breakthrough curves. The results show 0.257 min of space time is enough for a Ca(II) imprinted chitosan microspheres packed column to realize deep purification of water containing Pb(II). The Thomas rate constants are higher than 3 mL min-1 mg-1. The Modified dose-response model is feasible to predict the breakthrough curve. The reusability is confirmed during a multi-cycle adsorption-desorption process.