Oil separation from Putranjiva roxburghii seeds (non-edible urban waste) was carried out by solvent extraction method. The parameters of solvents suitability, extraction time, and aging of seeds were optimized in order to enhance the yield of bio-oil. The properties like acid value, calorific value, flash point, saponification value, iodine number, specific gravity, fire point, and kinematic viscosity were characterized. Bio-oil composition and functional groups were characterized using Gas Chromatography Mass Spectrometry (GC/MS) and Fourier Transform Infrared Spectroscopy (FT-IR). Maximum bio-oil yield of 43% was obtained with hexane at 61 degrees C and up to the time extent of 3.5 hours.