The supramolecular structure Ni(II) complex compound-DNA base-surfactant encapsulated within hybrid organic-inorganic silica nano-matrix has been obtained in a highly ordered form based on template synthesis of controlled sol-gel method. Some pre-defined properties of nano-matrix which depends on collective steric properties of supramolecular structure containing complex compound-DNA-surfactant according to the identification and using of an adequate surfactant for specific process of complex compound-DNA bonding have been investigated. The principal application deals at including supramolecular structure containing complex compound-DNA-surfactant in a silica nano-matrix, in order to obtain an intelligent material with optoelectronic properties.