In this paper, we demonstrate an easy approach - liquid crystal assisted replica molding method - to align uniaxial molecules in a patterned polymer at ambient temperature. During the process, the orientation of liquid crystals is parallel to the groove of the grating mold and the uniaxial molecules are aligned by liquid crystals. Many research groups in the field of optics and organic optoelectronics have paid huge attention to the polymer-chromospher system. Here, in order to quantify the degree of alignment, we measure both polarized absorption and photoluminescence (PL) spectra. According to our results, our approach to align molecules in a polymer can truly open up an easy way to manufacture optoelectronic and electronic devices.