We prepared maltose-polyrotaxane (Mal-PRX)-immobilized beads by 4 steps: (1) making polypseudorotaxane using amine-terminated poly(ethylene glycol)immobilized polystyrene (PEG-PS) beads and alpha-cyclodextrins, (2) capping the amine terminal of PEG in the polypseudorotaxane beads, (3) succination and (4) conjugating with maltose. The Mal-PRX-immobilized beads were mixed with biotinylated concanavalin A (Con A) to check the multivalent interaction with lectin. Quantum dot-streptavidin conjugate (QD-SV) was further introduced to enhance the fluorescence for the fluorescence microscope observation. The surface of Mal- PRX-immobilized beads became bright red by the emission 655 nm of QD-SV.