Particle self-assembly was affected by various factors. A theoretical formula using particle concentration, water evaporation rates, and withdrawal rates of substrate during evaporation-driven self-assembly had already reported: However, it showed a lack of evidence for large-size particles (more than diameter 2,106nm). Here, we suggest optimum fabrication conditions for large-size (3.0-m dia.) particle monolayer on a horizontally-set glass substrate by the evaporation-driven self-assembly. Two parameters, the volumes of used suspension and the meniscus angles between the suspension and the substrate, were examined. The experimental formula was also constructed by developing the reported formula.