Fullerene (C60) nanoparticles were assembled in two-dimension using a liquid-liquid interface. C60 nanoparticles dispersed in ethyl lactate/water solution were added to a glass vessel. Then, hexane was added to the dispersion solution to create the liquid-liquid interface. C60 nanocrystals were assembled at the interface, when ethanol was added to the water dispersion-hexane solution. The assembled film was transferred onto a solid substrate and the film morphology was observed by a scanning electron microscope (SEM) and atomic force microscope (AFM).