Cytoscreening of normal and cancer cells was performed using an infrared Raman spectroscopy together with data analysis software. The samples used were WFB (normal cell), W31 (cancer cell) and W14 (attenuated virulence cancer cell). Differential Raman spectra of cell samples were analyzed by using principal component analysis and discriminant analysis. Comparing the characteristic results, the increase of protein amide I, the significant decrease of lipid and the decrease of nucleic acid were observed. The accuracy of cell discrimination was around 93% for WFB, W31 and W14.