A rhodamine-based fluorescent chemosensor (Rh6G-2HC) was synthesized easily by a one-step condensation reaction. The structure was characterized using NMR, MS and EA spectroscopies. As soon as Cu2+ was added into the chemosensor solution, a color change from colorless to pink color was observed, and a considerable change in the absorption and fluorescence spectra. However, no spectral changes were indicated for other heavy metals such as Ag+, Zn2+, Cd2+, and Co2+. In order to provide a more detailed metal sensing performance, the optical and structural properties for the Cu2+ sensing effect were investigated using UV-Vis, fluorescence and H-NMR spectroscopies.