Colloidal microwires are constructed using anisotropic assembling templates with periodically wrinkled patterns of a liquid crystalline polymer (LCP). The wrinkled LCP patterns with the period of a few micrometers are produced from reactive mesogens on an alignment layer by the oxygen plasma treatment and used for assembling colloidal particles into microwires through a simple slit-coating process. A two-directional assembly of the colloidal particles is also demonstrated on the LCP template with two stamping-assisted rubbing directions. Our approach to the colloidal assembly is applicable for building up advanced photonic and optoelectronic devices.