CuO/Au@MWCNTs 나노복합재 기반 전기화학적 포도당 바이오센서의 민감도 개선

박미선; 배태성; 이영석; Mi-Seon Park; Tae-Sung Bae; Young-Seak Lee

Applied Chemistry for Engineering, Vol.27, No.2, 145-152, April, 2016

DOI10.14478/ace.2015.1117 Export Citation

CuO/Au@MWCNTs 나노복합재 기반 전기화학적 포도당 바이오센서의 민감도 개선

Improvement in Sensitivity of Electrochemical Glucose Biosensor Based on CuO/Au@MWCNTs Nanocomposites

박미선, 배태성^{1, †}, 이영석 ^†

충남대학교 대학원 바이오응용화학과
¹한국기초과학지원연구원

Mi-Seon Park, Tae-Sung Bae^{1, †}, and Young-Seak Lee^†

Department of Applied Chemistry and Biological Engineering, Chungnam National University, Daejeon 34134, Korea
¹Korea Basic Science Institute (KBSI) Jeonju Center, Jeonju 54907, Republic of Korea

E-mail:,

초록

본 연구에서는 전기화학적 바이오센서의 포도당 감지능을 높이고자 금 나노 입자가 분산된 다중벽탄소나노튜브 (multi-walled carbon nanotube, MWCNTs)에 CuO를 도입하였다. 금 나노 입자로 인하여 나노 클러스터(cluster) 형상을 갖는 CuO가 합성되었으며, 이는 포도당 감지능력에 매우 큰 영향을 나타내었다. 0.1 mole의 CuO가 합성되었을 때 CuO/Au@MWCNTs 나노복합재를 전극재료로서 바이오센서는 504.1 μA mM-1 cm-2으로 가장 높은 민감도를 보여주었으며, 이 값은 MWCNTs만을 전극으로 이용할 때보다 약 4배 정도 컸다. 또한, 0-10 mM의 긴 선형 구간(linear range)과 0.008 mM의 낮은 LoD (limit of detection) 값을 보여주었다. 이러한 실험 결과들은 CuO/Au@MWCNTs 나노복합재가 CuO를 이용한 다른 전기화학적 바이오센서보다 우수하다는 것을 입증하였으며, 이는 나노 클러스터 형상의 CuO가 포도당 감지에서 전기화학적 반응에 유리하기 때문으로 사료된다.

In this study, CuO was introduced on MWCNTs dispersed with Au nanoparticles to improve the glucose sensing capability of electrochemical biosensors. Nano-cluster shaped CuO was synthesized due to the presence of Au nanoparticle, which affects glucose sensing performance. The biosensor featuring CuO/Au@MWCNTs nanocomposite as an electrode material when 0.1 mole of CuO was synthesized showed the highest sensitivity of 504.1 μA mM-1 cm-2, which is 4 times better than that of MWCNTs based biosensors. In addition, it shows a wider linear range from 0 to 10 mM and lower limit of detection (LOD) of 0.008 mM. These results demonstrate that CuO/Au@MWCNTs nanocomposite sensors are superior to other CuO based biosensors which are attributed that the nano-cluster shaped CuO is favorable for the electrochemical reaction with glucose molecules.

Keywords:electrochemical glucose biosensor;non-enzymatic system;nanocomposite;copper oxide;multi-walled carbon nanotube

[References]

Jiang X, Wu Y, Mao X, Cui X, Zhu L, Sens. Actuators B-Chem., 153, 158 (2011)
Vashist SK, Zheng D, Rubeaan KA, Luong JHT, Sheu FS, Anal. Chim. Acta, 703, 124 (2011)
Zhang W, Ma D, Du J, Talanta, 120, 362 (2014)
Wang C, Huang H, Anal. Chim. Acta, 498, 61 (2003)
Tian X, Lian S, Zhao L, Chen X, Huang Z, Chen X, J. Solid State Electrochem., 18, 2375 (2014)
Lv X, Wang X, Huang D, Niu C, Zeng G, Niu Q, Talanta, 129, 20 (2014)
Park S, Boo H, Chung TD, Anal. Chim. Acta, 556, 46 (2006)
Hsu YW, Hsu TK, Sun CL, Nien YT, Pu NW, Ger MD, Electrochim. Acta, 82, 152 (2012)
Shervedani RK, Mehrjardi AH, Zamiri N, Bioelectrochemistry, 69, 201 (2006)
Wang L, Gao X, Jin L, Wu Q, Chen Z, Lin X, Sens. Actuators B-Chem., 176, 9 (2013)
Kong F, Gu S, Li W, Chen T, Xu Q, Wang W, Biosens. Bioelectron., 56, 77 (2014)
Lin KC, Lin YC, Chen SM, Electrochim. Acta, 96, 164 (2013)
Luo L, Zhu L, Wang Z, Bioelectrochemistry, 88, 156 (2012)
Zhuang ZJ, Su XD, Yuan HY, Sun Q, Xiao D, Choi MMF, Analyst, 133, 126 (2008)
Kumar SA, Cheng HW, Chen SM, Wang SF, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 30, 86 (2010)
Wang CX, Yin LW, Zhang LY, Gao R, J. Phys. Chem. C, 114, 4408 (2010)
Zheng XG, Xu CN, Tomokiyo Y, Tanaka E, Yamada H, Soejima Y, Phys. Rev. Lett., 85, 5170 (2000)
Chen J, Deng SZ, Xu NS, Zhang WX, Wen XG, Yang SH, Appl. Phys. Lett., 83, 746 (2003)
Chowdhuri A, Gupta V, Sreenivas K, Kumar R, Mozumdar S, Patanjali PK, Appl. Phys. Lett., 84, 1180 (2004)
You T, Niwa O, Tomita M, Ando H, Suzuki M, Hirono S, Electrochem. Commun., 4, 468 (2002)
Lee YS, Yoon KH, Carbon Lett., 16, 86 (2015)
Koo MY, Shin HC, Kim WS, Lee GW, Carbon Lett., 15, 255 (2014)
Saito T, Matsushige K, Tanake K, Physica B, 323, 280 (2002)
Chopra KL, Thin Film Phenomena, Wiley, New York (1969).
Im JS, Kim JG, Bae TS, Yu HR, Lee YS, Sens. Actuators B-Chem., 158, 151 (2011)
Wang J, Zhang WD, Electrochim. Acta, 56(22), 7510 (2011)
Wei H, Sun JJ, Guo L, Li X, Chen GN, Chem. Commun., 15, 2842 (2009)
Marioli JM, Kuwana T, Electrochim. Acta, 37, 1187 (1992)
Im JS, Yun J, Kim JG, Bae TS, Lee YS, Appl. Surf. Sci., 258(7), 2219 (2012)
Gougis M, Tabet-Aoul A, Ma D, Mohamedi M, Sens. Actuators B-Chem., 193, 363 (2014)
Adzic RR, Hsiao MW, Yeager EB, J. Electroanal. Chem. Interfacial. Electrochem., 260, 475 (1989)
Oliveira JE, Capparelli MLH, Medeiros ES, Zucolotto V, Biosensors, 2, 70 (2012)
Jiang LC, Zhang WD, Biosens. Bioelectron., 25, 1402 (2010)
Kawasaki T, Akanuma H, Yamanouchi TY, Diab. Care, 25, 353 (2002)
Vient B, Panzini B, Boucher M, Massicotte J, Clin. Chem., 44, 2369 (1998)
Reitz E, Jia WZ, Gentile M, Wang Y, Lei Y, Electroanalysis, 20, 2482 (2008)
Hajar S, Mahdi K, Ali AE, Chin. J. Catal., 34, 1208 (2013)

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