The cultural conditions for bacterial exopolysaccharide production by Agrobacterium radiobacter were optimised by response surface methodology (RSM) to maximise the viscosity of the broth and the yield of the polysaccharide. A study of complex interactions among sucrose concentration (0.5-5.5 g%), inoculum concentration(1-15%, v/v) and pH (4-8) was carried out using central composite design of experiments. The exopolysaccharide contained glucose. galactose and rhamnose (88.9:10.4:0.7) and formed an elastic gel on heating followed by cooling, at 2 g% concentration. The exopolysaccharide produced in a simpler inorganic nitrogen containing medium can be used as an emulsifier, viscosifier and also as a gelling agent in the preparation of microbiological media. The optimum conditions, which were obtained for maximum viscosity of the broth (105.64 mPas) and the yield of the polysaccharide (2.26%) were, sucrose concentration 2.62%,. inoculum concentration 1.0% and pH 6.24.