Process Biochemistry, Vol.37, No.1, 51-56, 2001
Optimisation of sucrose, inorganic nitrogen and abscisic acid levels for Santalum album L. somatic embryo production in suspension culture
An efficient process for the production of somatic embryos of sandalwood was developed by statistical optimisation of important medium constituents (viz. sugar, inorganic nitrogen and abscisic acid). Higher embryogenesis was observed after optimisation. The optimal levels for the constituents were, 37.56 g/l sucrose, 11.6 mM nitrate, 7.89 mM ammonium and 1.31 mg/l abscisic acid. Embryogenesis efficiency of 57.35% was achieved from experiments with media containing optimal levels of these four constituents. The correlation coefficient was 0.92 ensuring a satisfactory adjustment of the model to the experimental values.