Fomitopsis pinicola is used as a medicinal fungus in Asia. Polysaccharides extracted from 35-day-cultured fungus and its ethanolic extract showed no toxicity to endothelial cells (ECs). Extracted polysaccharides had a strong inhibitory effect on vascular endothelial growth factor (VEGF)-induced tube formation in ECs in a dose-dependent manner. Furthermore, the ethanolic extract dose-dependently suppressed production of the interferon (IFN)-gamma-induced inflammation marker, IP-10. High-molecular-weight of 5367 and 1056 kDa accounted for 19.6% and 19.3% of the total polysaccharides, and a low-molecular-weight one of 14.7 kDa accounted for 53.6% of the total polysaccharides. Chemical analyses of the polysaccharides revealed that myo-inositol, fucose, galactose, glucose, mannose, and fructose were neutral sugars in the polysaccharide. Six nucleoside-type compounds including cytidine, adenosine monophosphate (AMP), adenosine diphosphate (ADP), adenosine, inosine, and thymidine were identified in the ethanolic extract. These results suggest that different extracts from F pinicola play different roles in regulating the angiogenic process and inflammation. (C) 2008 Elsevier Ltd. All rights reserved.