In this study, we have developed a molecularly imprinted polymer (MIP) selective for (S)-propranolol that was encapsulated within an assembled gel, and allowed for an induced interaction in a live tissue environment. The development of the MIP microparticles having assembled nanoparticles with separated domains at their outer shell involved an assessment of the selectivity of the template and the morphology of its surface, using single and mixed crosslinking agents. The MIPs resulted in differential rebinding of the template, governed by different crosslinked architectures of the resulting polymer. The strength of the interaction of that shape molecule with the adaptable properties in the pore-wall of recognition site, especially an MIP that was prepared using a mixed crosslinker on lining silicone membrane, influenced high enantioselectivity in the gel. A different configuration response of the MIP embedded responsive gel enabled drug shaping, and affected generating the intrinsic recognition for an increase of the macromolecular proximity and dynamic interactions in the excised skin. Overall, the MIPs in a self-assembled microgel may show a potential for the study of integrated biological systems, with pharmaceutical and even therapeutic applications. (C) 2015 Elsevier Ltd. All rights reserved.