The separation of two different sized particles during evaporation of a dilute droplet is examined both computationally and experimentally. A transport model of the evaporating droplet system was solved using the finite element method to determine the fluid velocity, pressure, vapor concentration surrounding the droplet, temperature, and both particle concentrations. Experimentally, 1 m and 3 m polystyrene particles were used during the evaporation of a sessile water droplet. It was determined that to accurately model particle deposition, thermal effects need to be considered. The Marangoni currents in evaporating droplets keep particles suspended in the droplet until the end of the evaporation. Previous models of particle deposition during droplet evaporation have rapid accumulation of particles at the contact line. Our experiments and the experiments of others demonstrate that this is not accurate physically. In addition, to model the separation of two different sized particles the consideration of thermal effects is essential. (c) 2015 American Institute of Chemical Engineers AIChE J, 61: 3547-3556, 2015