화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.24, No.4, 336-341, April, 2016
DOI10.1007/s13233-016-4050-4  Export Citation
Wound healing effect of visible light-curable chitosan with encapsulated EGF
Shin-Hye Park1, 2, Eun-Hye Kim1, 3, Hyung-Jae Lee1, 3, Yun Heo1, 3, Young-Min Cho1, 3, Si-Yoong Seo1, 3, Tae-Yeon Kim4, Hyeun-Woo Suh4, Mi-Kyung Kim5, Yoshihiro Ito2, 6, Jae-Woon Nah7, and Tae-Il Son1, 3, †
  • 1Department of Systems Biotechnology, Chung-Ang University, Anseong, Gyeonggi, 17546, Korea
  • 2Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan
  • 3, Korea
  • 4Department of Plastic and Reconstructive Surgery, Bundang-Jesaeng-General Hospital, Seohyun-dong, Bundang-gu, Seongnam-si, Gyeonggi, 13590, Korea
  • 5Department of Pathology, College of Medicine, Chung-Ang University, Seoul, 06974, Korea
  • 6Department of Computational Intelligence and Systems Science, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan
  • 7Department of Polymer Science and Engineering, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam, 57940, Korea
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Low molecular O-carboxymethyl chitosan, a derivative of chitosan, was conjugated with a hydrophilic carboxymethyl group, following which the conjugate was modified with a furfuryl group. The chitosan derivative was cross-linked to encapsulate a model protein, bovine serum albumin (BSA), in the presence of Rose Bengal under visible light irradiation. The encapsulated BSA was slowly released from the matrix over 2 weeks. The modified chitosan exhibited antimicrobial activity and was non-cytotoxic to NIH3T3 fibroblasts. The wound healing effect of the cross-linked chitosan derivative was examined in Sprague-Dawley rats at 3, 7, and 14 days post-wounding. Cross-linked and murine epidermal growth factor (mEGF)-encapsulated chitosan derivatives healed wounds more rapidly in rats than non-encapsulated mEGF or chitosan derivative alone. Thus, visible light-curable chitosan has good potential for application as wound-healing matrix.
Keywords:chitosan;wound healing;protein encapsulation;epidermal growth factor;visible light-curable
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