An ionic liquid (IL) and double-stranded DNA (dsDNA) composite material was used to investigate the direct electron transfer of myglobin (Mb) on carbon ionic liquid electrode (CILE) The presence of the IL-dsDNA biocomposite film on the electrode surface provided great Improvement to the direct electron transfer rate of Mb with the CILE which was due to the synergistic contributions of specific characteristics of dsDNA IL and their interaction The electrochemical parameters of Mb in the IL-dsDNA composite film modified electrode were carefully investigated with the charge transfer coefficient (alpha) and the electron transfer rate constant (k(s)) calculated as 0 42 and 0 84s(-1) respectively The fabricated Mb modified electrode exhibited good electrocatalytic ability to the reduction of trichloroacetic acid and H(2)O(2) which showed the potential applications in the third-generation electrochemical biosensor (C) 2010 Elsevier B V All rights reserved