A vanadium pentoxide (V(2)O(5)) film was electrodeposited onto the end of a Ptlr wire (d = 125 mu m) via potential cycling in order to preliminarily assess its viability as a prospective material for in vivo H(2)S monitoring. The deposited oxide film exhibited catalytic activity towards the oxidation of H(2)S in a physiologically relevant pH buffer solution, which resulted in a large increase in peak current compared to that exhibited by the bare Ptlr surface. The modified electrode exhibited a linear response over a total sulfide concentration range of 0.5-15 mu M. The catalytic activity of the V(2)O(5)-modified wire towards the oxidation of upper physiological levels of NO and CO, two gaseous interferences found in vivo, was investigated using amperometry. While the electrode demonstrated some activity towards NO, it exhibited significant activity towards CO in the potential region of interest. However, the response towards NO and CO was much slower than the response towards H(2)S and the linear range remained unchanged despite the presence of the interfering gases. (C) 2011 Elsevier B.V. All rights reserved.