Solid silver mercury amalgam electrodes (AgSAEs) represent a suitable alternative to mercury electrodes due to their similar electrochemical properties and non-toxicity of the amalgam material. Based on this knowledge, we report the use of silver solid amalgam as working electrode to determine cefepime in human milk sample. Square-wave voltammogram registered for cefepime in Britton-Robinson buffer at pH 2.5, presented two well-defined reduction peaks, at -0.28 V (peak 1C) and -0.45 V (peak 2C). Under optimized condition, the calibration curves presented linear response from 3.0 x 10(-7) to 2.1 x 10(-6) mol L-1 with limits of detection and quantification of 8.51 x 10(-9) and 2.84 x 10(-8) mol L-1, respectively. The applicability of the method was verified using spiked human milk samples and testing recovery percentages, which were ranged from 98.00% to 100.5%. The obtained results were also compared with those provided with the HPLC technique. (C) 2012 Elsevier B.V. All rights reserved.