The electro-polymerization of L-Norvaline was investigated for the first time on glassy carbon electrode. The polymeric conditions and mechanism were discussed in detail. The electrochemical behaviors of metribuzin were studied systematically at this sensor and the dynamic parameters of electrode process were calculated. This electrochemical sensor, fabricated simply and very easy surface update, showed good response for metribuzin with a wide linear range from 6.43 x 10(-3) to 1.07 mu g/mL and a low detection limit of 2.14 x 10(-3) mu g/mL (S/N = 3). The proposed method was successfully applied to determine metribuzin in soil sample with satisfactory results. This work promoted the potential applications of amino acid materials in electrochemical sensors. (C) 2016 Elsevier B.V. All rights reserved.