The purpose of this work was to evaluate the expression of fatty acid metabolism-related genes in human adipose tissue from moderately obese women. We used qRT-PCR and Western Blot to analyze visceral (VAT) and subcutaneous (SAT) adipose tissue mRNA expression involved in de novo fatty acid synthesis (ACC1, FAS), fatty acid oxidation (PPAR, PPAR) and inflammation (IL6, TNF), in normal weight control women (BMI < 25 kg/m(2), n = 35) and moderately obese women (BMI 30-38 kg/m(2), n = 55). In SAT, ACC1, FAS and PPAR mRNA expression were significantly decreased in moderately obese women compared to controls. The downregulation reported in SAT was more pronounced when BMI increased. In VAT, lipogenic-related genes and PPAR were similar in both groups. Only PPAR gene expression was significantly increased in moderately obese women. As far as inflammation is concerned, TNF and IL6 were significantly increased in moderate obesity in both tissues. Our results indicate that there is a progressive downregulation in lipogenesis in SAT as BMI increases, which suggests that SAT decreases the synthesis of fatty acid de novo during the development of obesity, whereas in VAT lipogenesis remains active regardless of the degree of obesity.