At this time, a widely accepted model that can predict flow boiling heat transfer in microchannels with different fluids, geometries, and operative conditions is still missing. Depending on the working fluid, a predicting correlation can lead to accurate estimation or give rise to errors up to 50% and higher. The situation is further complicated when the working fluid is a zeotropic mixture of two components, due to the additional mass transfer resistance that must be estimated. In the recent years much attention has been paid to the possible use of fluorinated propene isomers in substitution for high-global-warming-potential refrigerants. The available hydrofluoroolefins cannot cover all the air-conditioning, heat pump, and refrigeration applications when used as pure fluids because their thermodynamic properties are not suitable for all the operating conditions, and therefore some solutions may be found using blends of refrigerants, to satisfy the demand for a wide range of working conditions. The adoption of new mixtures poses the problem of how to extend the correlations developed for pure fluids to the case of flow boiling of mixtures in microchannels. In this work, a mixture of R1234ze(E) and R32 (0.5/0.5 by mass) has been considered: The local heat transfer coefficient during flow boiling of this mixture in a single microchannel with 0.96mm diameter has been measured at a pressure of 14 bar, which corresponds to a bubble temperature of around 26 degrees C. This flow boiling database, encompassing more than 300 experimental points at different values of mass velocity, heat flux, and vapor quality, is compared with available correlations in the literature. The introduction of a correction to account for the additional mass transfer resistance is discussed, and such correction is found to be necessary and proper to provide a correct sizing of the evaporator.