Characterization of produced xylanase by Bacillus subtilis D3d newly isolated from apricot phyllosphere and its potential in pre-digestion of BSG

H. Moteshafi; M. Hashemi; S.M. Mousavi; M. Mousivand

Journal of Industrial and Engineering Chemistry, Vol.37, 251-260, May, 2016

DOI10.1016/j.jiec.2016.03.036 Export Citation

Characterization of produced xylanase by Bacillus subtilis D3d newly isolated from apricot phyllosphere and its potential in pre-digestion of BSG

H. Moteshafi, M. Hashemi^{1, †}, S.M. Mousavi^†, and M. Mousivand¹

Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University, Tehran, Iran
¹Department of Microbial Biotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research Education and Extension Organization (AREO), Karaj, Iran

E-mail:,

Bacillus subtilis D3d newly isolated from apricot phyllosphere has the potential of producing high levels of xylanase. The present study used response surface methodology (RSM) to optimize catalytic activity. Factors including temperature, pH and beechwood xylan concentration were optimized using central composite design (CCD). Under optimized condition maximum xylanase activity found 260 U/ml. The enzyme showed stability over a range of pH (5.0-9.0) and exhibited more than 80% activity after 72 h at 40 ℃. Such RSM models as well as stability data can be used at different operational conditions to predict the residual catalytic activity. Regard to pre-digested brewer’s spent grain (BSG) has potential as a valuable poultry B. subtilis D3d crude xylanase. Maximum reducing sugar yield were found to be 68.43 mgreducing sugars/gBSG at optimum condition. The results revealed that B.subtilis D3d is a promising xylanase-producing bacterium with great potential for use in the food and feed industries.

Keywords:Xylanase;Bacillus subtilis D3d;Brewer’s spent grain;Poultry feed;Response surface methodology

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[2] Xin FX, He JZ, Bioresour. Technol., 135, 309 (2013)

[3] Kim DH, Paik KH, J. Ind. Eng. Chem., 6(3), 194 (2000)

[4] Xue Y, Jameel H, Phillips R, Chang HM, J. Ind. Eng. Chem., 18(2), 707 (2012)

[5] Verma D, Satyanarayana T, Bioresour. Technol., 107, 333 (2012)

[6] Cherubini F, Energy Conv. Manag., 51(7), 1412 (2010)

[7] Polizeli MLTM, Rizzatti ACS, Monti R, Terenzi HF, Jorge JA, Amorim DS, Appl. Microbiol. Biotechnol., 67(5), 577 (2005)

[8] Martinez-Anaya MA, Jimenez T, Eur. Food Res. Technol., 205, 209 (1997)

[9] Shafisoltania M, Salehifara M, Hashemi M, Food Chem., 148, 176 (2014)

[10] Carvalho AFA, Neto PO, da Silva DF, Pastore GM, Food Res. Int., 51, 75 (2013)

[11] Beg QK, Kapoor M, Mahajan L, Hoondal GS, Appl. Microbiol. Biotechnol., 56(3-4), 326 (2001)

[12] Selle PH, Ravindran V, Partridge GG, Anim. Feed Sci. Technol., 153, 303 (2009)

[13] Hashemi M, Mousavi SM, Razavi SH, Shojaosadati SA, Ind. Crop. Prod., 43, 661 (2013)

[14] Mussatto S, J. Sci. Food Agric., 94, 1264 (2014)

[15] Schaad N, Jones JB, Chun W, Plant Pathol., 50, 812 (2001)

[16] Zhakaria MR, Tabatabaei M, Ghazali FM, Abd-Aziz S, Shirai Y, Hassan MA, World J. Microbiol. Biotechnol., 26, 767 (2010)

[17] Tamura K, Dudley J, Nei MM, Kumar S, J. Mol. Evol., 24, 1596 (2007)

[18] Kimura M, J. Mol. Evol., 16, 111 (1980)

[19] Fletcher M, J. Bacteriol., 170, 2027 (1998)

[20] Bailey M, Biely P, Poutanen K, J. Biotechnol., 23, 257 (1992)

[21] Maktouf S, Moulisb C, Kamoune A, Chaari F, Chaabouni SE, Remaud-Simeon M, Ind. Crop. Prod., 43, 349 (2013)

[22] Bernfeld P, Methods Enzymol., 1, 149 (1995)

[23] Poorna CA, Prema P, Biochem. Eng. J., 32, 106 (2006)

[24] Liao HP, Xu CM, Tan SY, Wei Z, Ling N, Yu GH, Raza W, Zhang RF, Shen QR, Xu YC, Bioresour. Technol., 123, 117 (2012)

[25] Deng P, Li DF, Cao YH, Lu WQ, Wang CL, Enzyme Microb. Technol., 39(5), 1096 (2006)

[26] Nagar S, Mittal A, Kumar D, Gupta V, Int. J. Biol. Macromol., 50, 414 (2012)

[27] Sa-Pereira P, Mesquita A, Duarte JC, Barros MRA, Costa-Ferreira M, Enzyme Microb. Technol., 30(7), 924 (2002)

[28] Kocabas DS, Ozben N, RSC Adv., 4, 26129 (2014)

[29] Wanga X, Luo H, Yu W, Ma R, You S, Liu W, Hou L, Zhenga F, Xie X, Yao B, Food Chem., 199, 516 (2016)

[30] Bajaj B, Manhas K, Biocatal. Agric. Biotechnol., 1, 330 (2012)

[31] Khattak FM, Pasha TN, Hayat Z, Mahmud A, J. Anim. Plant Sci., 16, 1 (2006)

[32] Waters D, Jacob F, Titze J, Arendt E, Zannini E, Eur. Food Res. Technol., 235, 767 (2012)

[33] Mussatto SI, Roberto IC, J. Chem. Technol. Biotechnol., 81(3), 268 (2006)

[34] Denstadli V, Westereng B, Biniyam H, Ballance S, Knutsen S, Svihus B, Br. Poult. Sci., 51(3), 419 (2010)

[35] Panwar D, Srivastava P, Kapoor M, Biocatal. Agric. Biotechnol., 3(2), 118 (2014)

[36] Maheshwari MU, Chandra TS, World J. Microbiol. Biotechnol., 16, 257 (2000)

[37] Phitsuwan P, Charupongrat S, Klednark R, Ratanakhanokchai K, J. Ind. Eng. Chem., 32, 360 (2015)

[38] Eom IY, Yu JH, J. Ind. Eng. Chem., 23, 72 (2015)