Direct Laser Interference Patterning (DLIP) is a versatile tool for the fabrication of micro and submicropatterns on different materials. In this work, DLIP was used to produce periodic surface structures on polyurethane (PU) substrates with spatial periods ranging from 0.5 to 5.0 tim. The influence of the laser energy density on the quality and topographical characteristics of the produced micropatterns was investigated. To characterize the surface topography of the produced structures, Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and Confocal Microscopy (CFM) were utilized. It was found that high quality and defect free periodic line-like patterns with spatial periods down to 500 nm could be fabricated, with structure depths between 0.88 up to 1.25 pin for spatial periods larger than 2.0 pin and up to 270 nm for spatial periods between 500 nm and 1.0 tim. Measurements of the contact angle of water on the treated surface allowed to identify an anisotropic wetting behavior depending mainly on the spatial period and filling factor of the structured surfaces. (C) 2015 Elsevier B.V. All rights reserved.