In the present investigation bulk algal biomass was collected from different natural water bodies of Himachal Pradesh, India. Maximum oil content of consortia was estimated at 3.7% and major fatty acids were oleic acid (18:1), palmitic acid (16:0), linoleic acid (18:2), and palmitoliec acid (16:1), which are the main constituents of biodiesel. Viscosity of algae oil was found to be high and transesterification was done by basic, microwave-, and ultrasonication-assisted methods. Maximum conversion from algal oil to fatty acid methyl esters was obtained by ultrasonication-assisted transesterification (71.5%), whereas microwave-assisted and basic transesterification was 58.6 and 51.3%, respectively.