화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Applied Chemistry for Engineering, Vol.27, No.3, 291-298, June, 2016
DOI10.14478/ace.2016.1035  Export Citation
아민 옥사이드 양쪽성 계면활성제 첨가가 리포좀 특성에 미치는 영향에 관한 연구
Effect of Amine Oxide Zwitterionic Surfactant on Characteristics of Liposome
모다희, 이수민, 이주연, 한동성1, 임종주†
  • 동국대학교-서울 공과대학 화공생물공학과
  • 1애경산업(주) 중앙연구소
DaHee Mo, SuMin Lee, JuYeon Lee, DongSung Han1, and JongChoo Lim†
  • Dept. of Chemical and Biochemical Engineering, Dongguk University-Seoul, Seoul, Korea
  • 1AK Central Research Center, DaeJeon, Korea
E-mail:
초록
본 연구에서는 pH 변화에 따른 양쪽성 계면활성제 첨가가 리포좀 특성에 미치는 영향에 관하여 살펴보았다. 이를 위하여 아민 옥사이드(amine oxide) 양쪽성 계면활성제를 합성하고 1H NMR, 13C NMR 및 FT-IR 분석을 통하여 구조를 규명하였으며, 계면활성제의 임계 마이셀 농도(critical micelle concentration, CMC) 및 표면장력 등의 계면 물성(interfacial property)을 측정하였다. 또한 제타 전위(zeta potential) 측정을 통하여 양쪽성 계면활성제가 양이온 계면활성제(cationic surfactant)에서 음이온 계면활성제(anionic surfactant)로 작용이 전환되는 등전점(isoelectric point)을 결정하였다. 이 결과를 바탕으로 등전점을 중심으로 한 pH 변화 및 아민 옥사이드 계면활성제의 탄화수소 사슬 길이가 리포좀의 평균 입자 크기 변화 및 제타 전위 등과 같은 안정성에 미치는 영향에 살펴보았다. 또한 pH 및 계면활성제의 탄화수소 사슬 길이 변화에 따른 및 리포좀과 계면활성제의 결합 정도(binding degree) 및 리포좀 막의 가변형성 (deformability) 등의 측정을 통하여 리포좀 막의 유동성(fluidity) 변화에 미치는 영향을 이해하고자 하였다.
In this study, zwitterionic surfactants were added to liposome systems at different pH conditions to understand the effect of surfactants on liposome characteristics. For this purpose, amine oxide surfactants having different hydrocarbon chain lengths were synthesized and the structure of the resulting product was elucidated by using 1H NMR, 13C NMR, and FT-IR. In addition, the physical properties of newly synthesized surfactants such as critical micelle concentration (CMC), surface tension and isoelectric point were measured. The stability characteristics of liposome systems including average particle sizes and zeta potentials were measured by varying pH and hydrocarbon chain lengths of an amine oxide surfactant. Effects of the pH and hydrocarbon chain length of an amine oxide surfactant on fluidity of a liposome membrane were also examined by measuring the deformability and the binding degree between the surfactant and liposome.
Keywords:amine oxide zwitterionic surfactant;liposome;deformability;stability
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