화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Chemical Engineering Research, Vol.54, No.3, 315-319, June, 2016
DOI10.9713/kcer.2016.54.3.315  Export Citation
통계학적 최적화를 이용한 아마란스 잎으로부터 폴리페놀 열수추출조건 최적화
Optimization of Extraction Conditions of Polyphenolic Compounds from Amaranth Leaf using Statistically-based Optimization
조재민, 최강훈, 신슬기, 이지현, 김진우†
  • 선문대학교 식품과학과, 31460 충남 아산시 탕정면 선문로 221번길 70
Jo Jaemin, Choi Kanghoon, Shin Seulgi, Lee Jihyun, and Kim JinWoo†
  • Department of Food Science, Sunmoon University, 70, Sunmoon-ro 221beon-gil, Tangjeong-myeon, Asan, Chungnam, 31460, Korea
E-mail:
초록
아마란스 종실 생산의 부산물인 아마란스 잎으로부터 폴리페놀 추출 증대를 위해 열수추출의 주요 공정조건인 추출시간, 추출온도와 에탄올 농도 중심합성법을 이용해 최적화하였다. 폴리페놀의 추출은 추출온도와 시간이 증가함에 따라 증가하였으며 추출에 에탄올 농도와 추출시간이 유의한 효과를 보였다(p<0.05). 열수추출의 에탄올 농도는 61.6 (v/v%) 에서 최대 폴리페놀 추출성능을 보이며 농도 증가에 따라 감소하는 경향을 보였다. 중심합성법에 의해 제시 된 2차 회귀방정식의 예측값과 실험값을 비교했을 때 매우 높은 합치도(R2=0.9566) 를 보였으며 추출온도 90.1 °C, 추출시간 50min과 에탄올 61.6 (v/v%) 공정조건에서 최대 농도인 12.6 mg GAE/g DM 폴리페놀을 얻을 수 있었다.
This study examined the optimization of hot-water extraction conditions for maximizing the total polyphenol compounds (TPC) extracted from amaranth leaf. The effects of three independent variables, including extraction temperature, extraction time and ethanol concentration on TPC were investigated using central composite design (CCD). The concentration of TPC increased with increased levels of extraction temperature and time. The extraction temperature and the ethanol concentration showed the significant effect on TPC production (p<0.05). The predicted values at the optimized condition were acceptable when compared to the experimental values (R2=0.9566). The optimum extraction conditions were as follows: temperature of 90.1 ℃, time of 50 min and ethanol concentration of 61.6% (v/v) for the maximum TPC of 12.6 mg GAE/g DM.
Keywords:Amaranth;byproduct;Polyphenol;Extraction;Optimization
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