2,4-Dinitroanisole (DNAN), a low sensitivity replacement for TNT, is a key component of a new class of melt cast formulations designed for use in insensitive munitions. It is therefore essential that its fate and transport in the environment be assessed before its large scale implementation. Several recent studies have described reductive biotransformation pathways leading to dead-end products. Recently a Nocardioides strain, JS1661 was isolated based on its ability to mineralize DNAN via the 2,4-dinitrophenol (DNP) pathway. However, its potential for degrading DNAN under environmentally relevant conditions was not examined. Therefore we evaluated the aerobic biodegradation of DNAN by JS1661 in non-sterile soil, aqueous media and in a fluidized bed bioreactor over a wide range of DNAN concentrations. DNAN was completely degraded under all tested conditions with little or no accumulation of DNP and almost stoichiometric release of nitrite. Furthermore, when DNAN was used as the sole carbon and nitrogen source, the accumulation of nitrite was dramatically reduced. The results of the study revealed the robustness of the strain over a range of loading rates in various physical environments suggesting that it could provide the basis for waste treatment, bioremediation and bioaugmentation applications. (C) 2016 Elsevier B.V. All rights reserved.