In this study, the effect of microstructural evolution of TiO2 nanotubular arrays on wettability and optical properties was investigated. Pure titanium was deposited on silica glass by PVD magnetron sputtering technique. The Ti coated substrates were anodized in an electrolyte containing NH4E/glycerol. The structures of the ordered anodic TiO2 nanotubes (ATNs) as long as 175 nm were studied using field emission scanning electron microscopy (FESEM) and X-ray diffraction (XRD). The result shows a sharp peak in the optical absorbance spectra around the band gap energy, 3.49-3.42 eV for annealed and non annealed respectively. The thermal process induced growth of the grain size, which influence on the density of particles and the index of refraction. Furthermore, the wettability tests' result displays that the contact angle of intact substrate (theta=74.7 degrees) was decreased to 31.4 degrees and 17.4 degrees after anodization for amorphous and heat treated (450 degrees C) ANTs coated substrate, respectively. (C) 2016 Elsevier Ltd. All rights reserved.