Melamine (Mel) is added illegally to foods and feeds due to its high nitrogen content and high intake, and it causes serious health problems, so it is urgent to build a simple and quick method for detecting Mel in our daily life. In this work, a self-powered Mel detection method is developed that is based on the newly invented triboelectric nanogenerator (TENG) by using the strong electronegativity property of Mel. In addition, Mel can enhance the electrical output signals of the TENGs by pairing with other triboelectric materials. Thus a high-sensitive Mel sensor is designed based on polytetrafl uoethylene (PTFE) TENG; the output current of TENG increases with the addition of Mel, resulting in largely improved detection limit of Mel with 0.5 ppb and a linear increase range from 1 to 500 ppb. This study not only develops a new type of material for TENG, but also demonstrates an innovative and convenient method for the detection of Mel and other biomaterials.