Nitrogen removal with different organic carbon effect was investigated using anaerobic baffled reactor ( ABR) anammox reactor. Results indicated that organic carbon exert an important effect on nitrogen removal through anammox process. When the feeding COD concentration was lower than 99.7 mg L (1), nitrogen removal could be enhanced via the coexistence of denitrification and anammox. Elevated COD could further deteriorate the anammox activity with almost complete inhibition at the COD concentration of 284.1 mg L (1). The nitrogen removal contribution rate of anammox was varied from 92.7% to 6.9%. However, the anammox activity was recovered when the COD/TN was decreased from 2.33 to 1.25 with influent nitrite addition. And, the anammox process was again intensified from 27.0 to 51.2%. High-throughput Miseq sequencing analyses revealed that the predominant phylum changed from Chloroflexi to Proteobacteria with the elevated COD addition, which indicated COD concentration was the most important factor regulating the bacterial community structure. (C) 2016 Elsevier Ltd. All rights reserved.