Bevacizumab (BEVA) is a monoclonal antibody (mAb) used in clinical oncology to treat certain types of metastatic cancers. The electrochemical oxidation of native and denatured BEVA was investigated in solution over a wide pH range and using BEVA-thin film voltammetry on a glassy carbon electrode. For native BEVA, only one pH-dependent oxidation peak, corresponding to tyrosine and tryptophan amino acid residues oxidation, was observed. The interfacial behaviour and adsorption of BEVA at the glassy carbon surface were evaluated by voltammetry and electrochemical impedance spectroscopy. The unfolding of the protein 3D morphological structure occurred upon denaturation with chemical agents, denaturing agent sodium dodecyl sulphate, and the reductants tris(2-carboxyethyl)phosphine and dithiothreitol, was electrochemically characterized. The electrochemical oxidation of denatured BEVA-thin film on GCE showed additional cysteine and histidine residues oxidation peaks. (c) 2016 Elsevier Ltd. All rights reserved.