In this paper, we have fabricated the samarium/gadolinium-substituted hydroxyapatite (Sm/Gd-HAP) coating on the borate-passivated AISI 316L stainless steel. The structural and morphological characteristics of the Sm/Gd-HAP coatings were investigated using various analytical techniques and the, anticorrosion behavior of the coating was studied through electrochemical techniques. The antibacterial activity of the coating was tested against Staphylococcus aureus; and Escherichia coli bacterial stains and, in particular, S. aureus showed better sensitivity toward the Sm/Gd-HAP coating, which might be due, to the Sin substitution in the coating. The in vitro cytotoxicity and biocompatibility of the as-developed coating on passivated AISI 316L SS were investigated using MC3T3-E1 cell Rue, which exhibited a high cell proliferation rate and provided significantly higher cell viability. Thus, the. resultant Sm/Gd-HAP coating on passivated AISI 316L SS could be considered:to be an effective bioimplant for bone tissue regeneration applications.