Twisted polymer microfiber/nanofiber yarns were manufactured by using a direct twisted electrospinning method. Compared with typical electrospinning setup, a significant difference in this method was that a high-speed motor was used to induce the spinneret tip with relatively short collecting distance at the electrospinning process. Two types of polymer self bundling yarns were prepared successfully: poly(vinylpyrrolidone) and poly(ether sulfones). The collecting distance was a very important factor to achieve twisted polymer microfiber/nanofiber yarns from ejected fiber bundles by this self-bundling and rotating electrospinning method. In addition, the possible mechanism for the self-bundling formation of twisted microfiber/nanofiber yarn was proposed.