New class of green nanostructured biocomposites was designed and synthesized for tissue engineering applications. These newly introduced non-cytotoxic and biodegradable polyurethanes are composed of linear aliphatic hexamethylene diisocyanate and polycaprolactone diol. Porosities of different synthesized biocomposite samples were 70-75 % for non-reinforced matrices, 64-70 % for reinforced with 5 wt% biocellulose nanofiber (BCNF), 59-69 % for 10 wt% of BCNF, and 56-69 % for 15 wt% of BCNF. Compressive strength results showed an increase of 84 % with BCNF reinforcement of 5 wt% and more than twofold increase (increase of 120 %) for 10 wt%. The tensile strength also increased up to almost twofold (115 %) for reinforcement with 5 wt% BCNF and to more than twofolds (143 %) for 10 wt% reinforcement. Higher degrees of reinforcement up to 15 wt% showed a detrimental effect on both properties. Results from biodegradation experiments showed that biocomposite scaffolds exhibited progressive mass loss over 5-week period, which varied according to the composition (ranged from 17 to 29 %), where the loss in mass was higher when soft segment content was higher.