Grooved and holed surface with a well fabricated design may serve as microsubstrate for cell culture. In this study, the author prepared chemically treated, micrometer scale grooved and holed glass surface by combination of chemical surface modification and ultraviolet (UV) excimer laser ablation technologies as follows ; Amine group attached glass surfaces, prepared by the treatment with an aminopropyltriethoxysilane, were condensed with a carboxylated radical initiator 4,4''-azobiscyanovaleric acid. Subsequently, poluacrylamide was grafted by surface initiated radical polymerization to create a very hydrophilic surface layer. Ultraviolet excimer laser beams (KrF : 248 nm) were irradiated through a microscope onto surfaces to create grooves or holes that were 10 and 50 micrometers in width and diameter, respectively. The depth, depending on the irradiation light strength, ranged from a few to several tenths of a micrometer. On eddothelial cell(EC) seeding, EC adhered and grew on the bottoms of the grooved or holed surface where native glass surface was exposed on ablation. Little cell adhesion was observed on nonablated, grafted surfaces. Endothelial ells aligned along the groove, resulting in very narrow tube like formation, whereas EC tended to form a multilayed spherical aggregates in a hole.