A new self-sustained fuel cell system was established using anodic TiO2/g-C3N4 and cathodic Pt nano catalysts. It is effective for pollutant Rhodamine B (RhB) removal in the alkaline anolyte (0.5 mol L-1 Na2SO4+ 0.5 mol L-1 NaOH). The cell voltage can reach similar to 0.6 V (1000 Omega). The anodic RhB degradation was affected by the system circuit connection modes (short-circuit, 1000 Omega and open-circuit). It was the most effective when an external 1000 Omega resistance was connected, and removed similar to 98% RhB in the anode chamber (350 mL, 10 mg L-1 RhB). Compared to the traditional method, it does not need any applied voltage or light irradiation to remove RhB. Cyclic voltammetry curves (CV) of TiO2/g-C3N4 anode in different electrolytes indicate excellent oxidation capacity at lower potential under alkaline than neutral condition. Presence of RhB under Ultraviolet light (UV) did not increase the system oxidation current as significantly as those without light under alkaline condition. The proposed mechanisms for RhB degradation were caused by the dark activation of O-2 and formation of reactive oxidizing species (ROS such as O-center dot(2)). It was confirmed by the electron spin resonance (ESR), even under open-circuit voltage (OCV) condition. The anodic TiO2/g-C3N4 catalyzed O-2 activation and pollutant oxidation. The RhB removal doesn't decrease as the temperature drops, because of the increase in dissolved oxygen (DO) and the formation of ROS. To cold season, this is a significant advantage compared with microbial methods for practically wastewater treatment. (C) 2016 Elsevier Ltd. All rights reserved.